DESCRIPTION:
Convenient,costeffectivesamplerpackcontainingfourDPc10peptidederivatives(200µgeach)asfollows:
- DPc10Peptide(Cat.A010-514)
- DPc10InactivePeptide(Cat.A010-515)
- FluoresceinDPc10Peptide(Cat.A010-516)
- FluoresceinDPc10InactivePeptide(Cat.A010-517)
DPc10peptideisa36-residuepeptidecorrespondingtotheGly2460-Pro2495regionofrabbitRyR2.ItinteractswithRyR2andprovokesabnormalchannelactivityusuallyseeninmutantRYR2channelsassociatedwithCPVT.ThispeptidecanbeusedtoprovokepathologicalRyR2behaviourinwild-typeRyR2channels.ThepeptidebindsataninterfacebetweendomainsofRYR2,“unzipping”thesedomainsandalteringchannelfunction.TheDPc10bindingsiteispreservedinRyR2fromallmammalianspecies.DPc10(0.5-5µM)elevatesCa2+-leak,increasesCa2+-sparkactivity,ispro-arrhythmic,andprovokeshypertrophyincardiacmyocytes.
DPc10inactivepeptideisa36-residuepeptidethatdiffersfromDPc10(Gly2460-Pro2495,rabbit)atoneposition(R2475S)andrepresentsthesequenceofaknownCPVTmutantRyR2domain.ThispeptidebindsweaklyorfailstobindtotheDPc10interfaceanddoesNOTaffectchannelfunction.ItservesasanegativecontrolforresearchprojectsusingDPc10peptide.
DPc10peptidesinthecurrentformarenotcellpermeable.TheproductsareusefulinapplicationsthatpermitaccesstoRyR2directly(viawholecellpatch,skinnedcells,blacklipidmembrane,vesicles,orupondeliverytocytoplasm).
ReadDPc10Publications
DATASHEET:
Solubilisation | DMSO |
Storage | Storeassuppliedat-20°Cforupto1year.Storedesiccated.Makefreshsolutionspriortouse. |
Datasheet | A010-518ReadDPc10Publications |
MSDS | A010-518 |
Notes | Theproductswerepurifiedinacetonitrileandwatercontaining0.1%trifluoroaceticacid(TFA)priortolyophilisation.Theproductswillthereforebepresentasitstrifluoroaceticacidsalts. *Peptidesaresuppliedbynetpeptideweight.Whilstthepurityofthematerialhasbeenshowntobe>95%,thenetpeptidecontentislowerduetocounterionsandresidualwaterpresentinthematerial.Tocompensateforthistheamountofpeptideweighedoutisadjustedsothattheamountofactivematerialreceivedisexactlyasstatedonthevial.Thismeansthatthetotalmasswillbeinexcessof200µg,buttherewillbeexactly200µgoftheactivepeptideinthevial.Theremainderoftheweightisduetothecounterionsandresidualwater. |
Regulatorystatement | Productforresearchuseonly.Notintendedfordiagnosticortherapeuticuse. |