DESCRIPTION:
5Flu-GFCPDHKAAMVLFLDRVYGIEVQDFLLHLLEVGFLP
FluoresceinderivativeofDPc10peptide.
DPc10peptideisa36-residuepeptidecorrespondingtotheGly2460-Pro2495regionofrabbitRyR2.ItinteractswithRyR2andprovokesabnormalchannelactivityusuallyseeninmutantRyR2channelsassociatedwithCPVT.ThispeptidecanbeusedtoprovokepathologicalRyR2behaviourinwild-typeRYR2channels.ThepeptidebindsataninterfacebetweendomainsofRyR2,“unzipping”thesedomainsandalteringchannelfunction.TheDPc10bindingsiteispreservedinRyR2fromallmammalianspecies.
DPc10(0.5-5µM)elevatesCa2+-leak,increasesCa2+-sparkactivity,ispro-arrhythmic,andprovokeshypertrophyincardiacmyocytes.
DPc10inthecurrentformisnotcellpermeable.TheproductisusefulinapplicationsthatpermitaccesstoRYR2directly(viawholecellpatch,skinnedcells,blacklipidmembrane,vesicles,orupondeliverytocytoplasm).
ReadDPc10Publications
DATASHEET:
Peptidename | FluoresceinDPc10Peptide |
Peptidesequence | 5Flu-GFCPDHKAAMVLFLDRVYGIEVQDFLLHLLEVGFLP |
N-terminus | 5-Carboxyfluorescein |
C-terminus | Acid |
Counterions | Trifluroacetate(TFA) |
Molecularweight | 4462.155 |
HPLCanalysis | Analysiscarriedoutusinga100A4.6x150mmcolumn,grADIentfrom10%-90%Acetonitrile,in15minutes.60degC.Puritydeterminedas95%. |
MSanalysis | Molecularionpredicted:4462.155.Observed:4461.3[M+3H] |
Appearance | Yellowpowderedsolid |
Solubilisation | DMSO |
Storage | Storeassuppliedat-20°Cforupto1year.Storedesiccated.Makefreshsolutionspriortouse. |
Datasheet | A010-516ReadDPc10publications |
MSDS | A010-516 |
Notes | Theproductwaspurifiedinacetonitrileandwatercontaining0.1%trifluoroaceticacid(TFA)priortolyophilisation.Theproductwillthereforebepresentasitstrifluoroaceticacidsalt. *Peptideissuppliedbynetpeptideweight. Whilstthepurityofthematerialhasbeenshowntobe>95%,thenetpeptidecontentislowerduetocounterionsandresidualwaterpresentinthematerial. Tocompensateforthistheamountofpeptideweighedoutisadjustedsothattheamountofactivematerialreceivedisexactlyasstatedonthevial. Thismeansthatthetotalmasswillbeinexcessof200µg,buttherewillbeexactly200µgoftheactivepeptideinthevial.Theremainderoftheweightisduetothecounterionsandresidualwater. |
Regulatorystatement | Productforresearchuseonly.Notintendedfordiagnosticortherapeuticuse. |