DESCRIPTION:
LyophilisedrabbitpolyclonalproteinAaffinitypurifiedantibody
GFPwasintroducedasatoolformolecularBIOLOGyover20yearsagoandisthoughtofasoneofthosemostsignificantcontributionstomodernmicroscopy.Manyrelatedfluorescentproteinsaresubjecttooligomerisationandphotobleachingandassuch,limittheresolutionobtainablefromtheiruse.mEOS2isaphotoactivatablegreentoredphotoconvertIBLefluorescentproteinderivedfromAnthozoa.Ithasrecentlyemergedandissettomakesignificantcontributionstotheenhancementofsuperresolutionmicroscopyforthestudyofcellularultrastructureandproteintrafficking.TherearealreadyanumberofpapersdescribingitsuseinthedevelopingfieldofPhotoactivatedlocalisationmicroscopy(PALM)reversiblesaturableopticalfluorescencetransitions(RESOLFT),stochasticopticalreconstructionmicroscopy(STORM)andthree-dimensionalhigh-resolutionimaging(McKinneyetal.2009).A010-mEOS2isaproteinAaffinitypurifiedrabbitpolyclonalantibodyraisedagainstpurifiedmEOS2expressedinE.coliandstainsmEOS2fusionproteinsexpressedinbacteriaandmammaliancellsinbothwesternblottingandimmunofluorescencemicroscopyapplications.
DATASHEET:
Description | LyophilisedrabbitpolyclonalproteinAaffinitypurifiedantibody |
Immunogen | PurifiedmEOS2expressedasaGSTfusionproteininBL21RosettaE.colicells |
Isotype | IgG |
Purification | ProteinAaffinitypurified |
Speciesreactivity | N/A |
Testedapplications | WB |
Recommendeddilution | WB1:10,000forhighexpression,1:1000forlowlevelexpression |
Optimization | Optimaldilutionstobedeterminedbyenduser |
Storage | Lyophilisedantibodyisstableat4°Cwhenstoredwithdesiccant.Reconstitutelyophilisedpowderin50µlof18MΩH2Ofora1mg/mlsolutionwithstablebufferingconditions.Aliquotandstorefrozenat-80°Cfor1year.Avoidfreeze-thawcycles. |
Storage | Lyophilised4°CReconstituted-80°C |
Datasheet | A010-mEOS2 |
MSDS | A010-mEOS2 |
Regulatorystatement | Productforresearchuseonly.Notintendedfordiagnosticortherapeuticuse. |