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Badrilla/Green fluorescent protein (GFP, EGFP) pAb epitope purified/A010-pGFP-50/50 μg (312 blots)
DESCRIPTION:
Afullyvalidated,highlyspecific,affinitypurifiedrabbitpolyclonalantibodytoGFP,EGFP,PA-GFP,CFPandYFP
Background:Thegreenfluorescentprotein(GFP)iscomposedof238aminoacidresidues(26.9kDa)andexhibitsbrightgreenfluorescencewhenexposedtoultravioletbluelight.IthasbecomeawellestablishedreporterforgeneexpressionandMarkerforproteintargeting.SubtlemodificationshavebeenmadetothesequenceofGFPimprovingitssolubilityandphotostablility(eGFP)toallowacquisitionofmorecomplexdata(FRET,FRAP).Moreover,subtleresiduemodificationshavebeenengineeredtoaltertheemissionwavelengthsuchasinCFP,YFPtofacilitateduellabelling.Anti-GFPAbisapolyclonalantibodyraisedinrabbitbyimmunisationwith,andaffinitypurificationagainstfulllengthGFP(Faixetal.,2001).ItproducesexceptionalstainingforGFP,PA-GFP,EGFP,CFPandYFPinwesternblottingatadilutionof1:10,000andbioimagingapplicationsathighdilutions,ithasfeaturedinanimpressivenumberofpublications.
TestedApplications:Westernblot(1:10,000dilution),IFMicroscopy(1:500-5000dilution)
DATASHEET:
Description | LyophilisedrabbitpolyclonalmonoclonalantibodytoGFP |
Immunogen | RecombinantGFPtogetherwithcompleteFreund"sadjuvant |
Isotype | IgG |
Purification | AffinitypurifiedusingpurifiedGFPcovalentlycoupledtocyanogenbromideactivatedagarose. |
Speciesreactivity | Willrecogniseregular,enhancedandphotoactivatablegreenfluorescentproteinsGFP,EGFP,PAGFP.ItwillalsorecognisecyanandyellowfluorescentproteinsCFP,YFP,butwillnotrecogniseanyredfluorescentproteins. |
Testedapplications | WB,IF,IP,ELISA |
Recommendeddilution | WB1:10,000,IF1:500-5000,IP,ELISA |
Optimization | Optimaldilutionstobedeterminedbyenduser |
Storage | Lyophilisedantibodyisstableat4°Cwhenstoredwithdesiccant.Reconstitutelyophilisedpowderin6.25µlof18MΩH2O,aliquotandstorefrozenat-80°Cfor1year.Avoidfreeze-thawcycles. |
Storage | Lyophilised:4°C.Reconstituted:-80°C |
Datasheet | A010-pGFP-50 |
MSDS | A010-pGFP-50 |
Regulatorystatement | Productforresearchuseonly.Notintendedfordiagnosticortherapeuticuse. |