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Badrilla/绿色荧光蛋白(GFP,EGFP)pAb表位纯化/A010-pGFP-5/5μg-足够31个杂交
DESCRIPTION:
Afullyvalidated,highlyspecific,affinitypurifiedrabbitpolyclonalantibodytoGFP,EGFP,PA-GFP,CFPandYFP
Background:Thegreenfluorescentprotein(GFP)iscomposedof238aminoacidresidues(26.9kDa)andexhibitsbrightgreenfluorescencewhenexposedtoultravioletbluelight.IthasbecomeawellestablishedreporterforgeneexpressionandMarkerforproteintargeting.SubtlemodificationshavebeenmadetothesequenceofGFPimprovingitssolubilityandphotostablility(eGFP)toallowacquisitionofmorecomplexdata(FRET,FRAP).Moreover,subtleresiduemodificationshavebeenengineeredtoaltertheemissionwavelengthsuchasinCFP,YFPtofacilitateduellabelling.Anti-GFPAbisapolyclonalantibodyraisedinrabbitbyimmunisationwith,andaffinitypurificationagainstfulllengthGFP(Faixetal.,2001).ItproducesexceptionalstainingforGFP,PA-GFP,EGFP,CFPandYFPinwesternblottingatadilutionof1:10,000andbioimagingapplicationsathighdilutions,ithasfeaturedinanimpressivenumberofpublications.
TestedApplications:Westernblot(1:10,000dilution),IFMicroscopy(1:500-5000dilution).
DATASHEET:
Description | LyophilisedeabbitpolyclonalmonoclonalantibodytoGFP |
Immunogen | RecombinantGFPtogetherwithcompleteFreund"sadjuvant |
Isotype | IgG |
Purification | AffinityPurifiedusingpurifiedGFPcovalentlycoupledtocyanogenbromideactivatedagarose |
Speciesreactivity | No |
Testedapplications | WB,IF,IP,ELISA |
Recommendeddilution | WB1:10,000,IF1:500-5000,IP,ELISA |
Optimization | Optimaldilutionstobedeterminedbyenduser |
Storage | Lyophilisedantibodyisstableat4°Cwhenstoredwithdesiccant.Reconstitutelyophilisedpowderin6.25µlof18MΩH2O,aliquotandstorefrozenat-80°Cfor1year.Avoidfreeze-thawcycles. |
Storage | Lyophilised:4°C.Reconstituted:-80°C |
Datasheet | A010-pGFP-5 |
MSDS | A010-pGFP-5 |
Regulatorystatement | Productforresearchuseonly.Notintendedfordiagnosticortherapeuticuse. |