DESCRIPTION:
Avalidated,affinitypurifiedmousemonoclonalantibodyclone6B2tonitrotyrosine
Alargenumberofphysiologicalandpathologicaleventsleadtosocalledstressconditionstowhichtheorganismcanadaptwithincertainlimitsbyregulatingtheactivityofaseriesofenzymaticcascades.Moststressresponsesarecharacterizedbyanincreasedgenerationof“freerADIcals”whichencompassbothreactiveoxygen(ROS)andnitrogenspecies(RNS).Mostofthesespeciesreactwithmacromoleculesoftheorganism,i.e.aminoacids,proteins,lipidsandDNA,leadingtofunctionalalterationswhichcaneitherparticipatetoadaptationorleadtocelldeath.
Oxidativestresshowever,hasdifferentfunctionalconsequencesdependingnotonlyuponitsintensityandduration,butalsouponthenatureofthefreeradicals,ROSorRNS,generated.Interestingly,thenatureofthesereactivespeciesdependsontheratiobetweentheinitiallyproducedROS,superoxideanion(O2.-),andnitricoxide(NO).Indeed,O2.-veryrapidlyreactswithNOandtherefore,aslongastheNO/O2.-ratiois=1,O2.-willthereforepreferentiallyreactwithNOratherthanwithmacromolecules,thusgeneratingreactivenitrogenspecies(RNS):nitrosonium(NO+),N2O3andperoxinitrite(ONOO-).TheseRNSinduceposttranslationalmodifications:forNO+andN2O3,S-nitrosation(Cys-SNO)andforONOO-tyrosinenitration(Tyr-NO2),methioninesulfoxidation(Met-SOH)andthioloxidation(RS-S-R).WhentheO2./NOratiobecomes>1,theO2.-andNO.2ionsandthereaftertheOH.radicalscauseirreversIBLeoxidationsandperoxidationsofmacromoleculeswhichgenerallyleadtocelldeath(1-4).ThemonoclonalantibodieshavebeenselectedfortheirhighaffinityandspecificitytowardsTyr-NO2residues.Theydonotcross-reactwithTyrorTyrderivativessuchasaminotyrosine,chlorotyrosineorphosphotyrosine,neitherwithnitroTrpwhichcanalsobegeneratedinresponsetoperoxynitrite.TheyalsorecognizeTyr-NO2residuesinvarioussequencesasshownbytheirABIlitytorecognizenitratedproteinsincludingalbumin,ovalbumin,insulin,hemoglobin,KLHandvariouscytoplasmicandmitochondrialproteins.
TestedApplications:Westernblot(1:5000dilution).Reactivity:ReactswithNitrotyrosineeitherfreeorincorporatedinproteinssuchashumanserumnitroalbumin,nitrohaemoglobinandnitroinsulin.Allspecies
DATASHEET:
| Description | LyophilisedmousemonoclonalaffinitypurifiedantibodycontainingIgG1antibodyspecificfornitrotyrosine. |
| Immunogen | Protein-boundnitrotyrosine |
| Isotype | No |
| Purification | ProteinAaffinitypurified |
| Speciesreactivity | Nitrotyrosineeitherfreeorincorporatedinproteinssuchashumanserumnitroalbumin,nitrohaemoglobinandnitroinsulin.Allspecies. |
| Testedapplications | WB |
| Recommendeddilution | WB1:5000 |
| Optimization | Optimaldilutionstobedeterminedbyenduser |
| Storage | LyophilisedA010-6B2(20µl)inPhosphateBufferedSaline10mM,NaCl0.15M(pH7.4)Thimerosal0,01%maybeusedaspreservative. |
| Storage | Lyophilised:4°C.Reconstituted:-80°C |
| Datasheet | A010-6B2AP |
| MSDS | A010-6B2AP |
| Regulatorystatement | Productforresearchuseonly.Notintendedfordiagnosticortherapeuticuse. |
