DESCRIPTION:
Avalidated,affinitypurifiedmousemonoclonalantibodyclone2E11tonitrotyrosine
Age-relateddeclineofcardiacperformanceisoftenassociatedwithoxidativestressandincreasedlevelsoffreerADIcals,encompassingbothreactiveoxygen(ROS)andnitrogenspecies(RNS).FormationofRNSspeciesisreportedinacutemyocardiacdisorderssuchasheartfailure.Thesespeciesaremetabolitesand/oroxidationproductsofnitrogenmonoxide(NO),suchasnitrogendioxide(NO2)andperoxynitrite(ONOO).Ahallmarkofthesespeciesistheconversionoftyrosineto3-nitrotyrosine(3-NY).
Badrilla"smonoclonalantibodieshavebeenselectedfortheirhighaffinityandspecificitytowardsNYresidues.Theydonotcross-reactwithtyrosineortyrosinederivativessuchasaminotyrosine,chlorotyrosineorphosphotyrosine,neitherwithnitroTrpwhichcanalsobegeneratedinresponsetoperoxynitrite.TheyalsorecognizeNYresiduesinvarioussequences,asshownbytheirABIlitytorecognisenitratedproteinsincludingalbumin,ovalbumin,insulin,hemoglobin,KLHandvariouscytoplasmicandmitochondrialproteins.
Testedapplications:Westernblot(1:2000dilution). ReactswithNitrotyrosineeitherfreeorincorporatedinproteinssuchashumanserumnitroalbumin,nitrohaemoglobinandnitroinsulin.
DATASHEET:
| Description | Lyophilisedmousemonoclonalaffinitypurifiedantibody(A010-2E11AP)containingIgG2bantibodyspecificfornitrotyrosine. |
| Immunogen | Protein-boundnitrotyrosine |
| Isotype | No |
| Purification | ProteinAaffinitypurified |
| Speciesreactivity | Nitrotyrosineeitherfreeorincorporatedinproteinssuchashumanserumnitroalbumin,nitrohaemoglobinandnitroinsulin.Allspecies. |
| Testedapplications | WB |
| Recommendeddilution | WB1:2000 |
| Optimization | Optimaldilutionstobedeterminedbyenduser |
| Storage | Lyophilisedantibodyisstableat4°Cwhenstoredwithdesiccant.Reconstitutelyophilisedpowderin20µlof18MΩH2O,aliquotandstorefrozenat-80°Cfor1year.Avoidfreeze-thawcycles. |
| Storage | Lyophilised:4°C.Reconstituted:-80°C |
| Datasheet | A010-2E11 |
| MSDS | A010-2E11 |
| Regulatorystatement | Productforresearchuseonly.Notintendedfordiagnosticortherapeuticuse. |

