DESCRIPTION:
Afullyvalidated,highlyspecificaffinitypurifiedrabbitpolyclonalantibodytoαandβisoformsofthreonine-286phosphorylatedCaMKII
Ca2+/calmodulin-dependentkinaseII(CaMKII)isaubiquitous,multifunctionalserine/threoninekinaseinvolvedintranslatingCa2+-transientsignalsintocellularresponses.FourseparateCaMKIIgenesareexpressedinman(α,β,δ,γ)withisoformswithineachgene.CaMKIIδandγareabundantintheheart.CaMKIIisactivatedbyCa2+/CaMbinding,andbecomesactivatedforprolongedperiodsoftimefollowingautophosphorylationofThr-286orThr-287(equivalentresiduesindifferentisoforms).Thisantibodyrecognizestheactive,autophosphorylatedenzyme(allisoforms)andthuscanmeasuretheabundanceofactiveCaMKII.ProlongedactivationofCaMKIIisassociatedwithavarietyofvascularandcardiacdiseasesincludingstroke,atrialfibrillationcardiacarrhythmia&suddendeath,cardiacapoptosis,andheartfailure.
Antibodyform:LyophilisedProteinAAffinityPurifiedAntibody(IgG). Speciesrecognition:rat,humanandmouse.Expectedtoreactwithvariousisoformsfromchicken,cow,pig,rabbit,orangutan,zebrafish,ferretandXenopuslaevis.
DATASHEET:
Description | AffinitypurifiedrabbitpolyclonalantibodytoCaMKIIphosphothreonine-286/287 |
Immunogen | Syntheticpeptide(M281HRQET(PO3H2)VDC289)correspondingtoaminoacidssurroundingthephosphorylatedthreonineresidueatposition286or287ofCaMKII,whichwasconjugatedtokeyholelimpethemocyanin(KLH)bycarbodiimidecrosslinkage. |
Isotype | IgG |
Purification | ProteinAaffinitypurified |
Speciesreactivity | Reactswithrat.Notyettestedinotherspecies.However,fromprimarysequenceanalysis,theantibodywillrecognisePhosphoThr-286(or287)inallisoforms(α,β,γandδ)fromhuman,ratandmouse.Theantibodyisalsoexpectedtoreactwithvariousisoformsfromchicken,cow,pig,rabbit,orangutan,zebrafish,ferretandXenopuslaevis. |
Testedapplications | WB |
Recommendeddilution | WB1:1000 |
Optimization | Optimaldilutionstobedeterminedbyenduser |
Storage | Lyophilisedantibodyisstableat4°Cwhenstoredwithdesiccant.Reconstitutelyophilisedpowderin50µlof18MΩH2O,aliquotandstorefrozenat-80°Cfor1year.Avoidfreeze-thawcycles. |
Storage | Lyophilised:4°C.Reconstituted:-80°C |
Datasheet | A010-50AP |
MSDS | A010-50AP |
Regulatorystatement | Productforresearchuseonly.Notintendedfordiagnosticortherapeuticuse. |