DESCRIPTION:
Afullyvalidated,highlyspecificrabbitpolyclonalantibodytoαandβisoformsofCaMKIIPhosphoThr-286(orThr287inβ)
Ca2+/calmodulin-dependentkinaseII(CaMKII)isaubiquitous,multifunctionalserine/threoninekinaseinvolvedintranslatingCa2+-transientsignalsintocellularresponses.FourseparateCaMKIIgenesareexpressedinman(α,β,δ,γ)withisoformswithineachgene.CaMKIIδandγareabundantintheheart.CaMKIIisactivatedbyCa2+/CaMbinding,andbecomesactivatedforprolongedperiodsoftimefollowingautophosphorylationofThr-286orThr-287(equivalentresiduesindifferentisoforms).Thisantibodyrecognizestheactive,autophosphorylatedenzyme(allisoforms)andthuscanmeasuretheabundanceofactiveCaMKII.ProlongedactivationofCaMKIIisassociatedwithavarietyofvascularandcardiacdiseasesincludingstroke,atrialfibrillationcardiacarrhythmia&suddendeath,cardiacapoptosis,andheartfailure.
Testedapplications: westernblot(1:5000dilution) Speciesrecognition: rat,humanandmouse.Expectedtoreactwithvariousisoformsfromchicken,cow,pig,rabbit,orangutan,zebrafish,ferretandXenopuslaevis.
DATASHEET:
Description | Lyophilisedrabbitpolyclonalanti-serumtoCaMKIIPhosphoThr-286/287. |
Immunogen | Syntheticpeptide(M281HRQET(PO3H2)VDC289)correspondingtoaminoacidssurroundingthephosphorylatedthreonineresidueatposition286(or287)ofCaMKII,whichwasconjugatedtokeyholelimpethemocyanin(KLH)bycarbodiimidecrosslinkage. |
Isotype | IgG |
Purification | Rawserum |
Speciesreactivity | Reactswithrat.Notyettestedinotherspecies.However,fromprimarysequenceanalysis,theantibodywillrecognisePhosphoThr-286(or287)inallisoforms(α,β,γandδ)fromhuman,ratandmouse.Theantibodyisalsoexpectedtoreactwithvariousisoformsfromchicken,cow,pig,rabbit,zebrafish,andXenopuslaevis |
Testedapplications | WB |
Recommendeddilution | WB1:5000 |
Optimization | Optimaldilutionstobedeterminedbyenduser |
Storage | Lyophilisedantibodyisstableat4°Cwhenstoredwithdesiccant.Reconstitutelyophilisedpowderin20µlof18MΩH2O,aliquotandstorefrozenat-80°Cfor1year.Avoidfreeze-thawcycles. |
Storage | Lyophilised:4°CReconstituted:-80°C |
Datasheet | A010-50 |
MSDS | A010-50 |
Regulatorystatement | Productforresearchuseonly.Notintendedfordiagnosticortherapeuticuse. |