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Badrilla/Ryanodine受体2(RYR2)(去磷2808)pAb/A010-35AP/100μl
DESCRIPTION:
Afullyvalidated,highlyspecificaffinitypurifiedrabbitpolyclonalantibodytoSerine-2808dephosphorylatedRyanodineReceptor
TheRyanodineReceptor(RyR2)isaCa2+channelofcardiacmusclethatplaysacentralroleinECcoupling.ThebindingofCa2+toryanodinereceptor2RyR2opensthechannelandCa2+ storedintheSRmovesthroughthechannelintothecytosoltoinitiatemusclecontraction.Abnormalstructureandfunctionofryanodinereceptorshasbeenreportedinfailinghearts,withSer-2809phosphorylationappearingelevatedinclinicalsituationswhichmaycontributetotheabnormalCa2+ handlingcharacteristicsofcardiacmuscleintheseconditions.Serine-2809canbephosphorylatedbyPKAorCaMKII,whichiscoincidentwithsignificantchangeinRyR2channelfunctiontypifiedbyanincreasedopenprobABIlity, theabrogationoftheinhibitoryeffectsofCaM andMg2+,dissociationofregulatoryfactors,expressionofsubconductancestatesandtheexpressionofchannelactivityatdiastolicCa2+concentrations. ThisantibodyrecognisesDephosphorylatedSer-2808(humansequence)orSer-2809fromrabbit.
Testedapplications:Westernblot(1:5000dilution),IHCMicroscopy(1:100).Speciesrecognition:allmammalianspecies,whendephosphorylatedonSer-2808(or2809rabbit).
DATASHEET:
Description | AffinitypurifiedRabbitpolyclonalantibody(A010-35)todephosphorylatedryanodinereceptorSer-2808 |
Immunogen | Syntheticpeptide(Y2802NRTRRISQT2811)correspondingtoaminoacidssurroundingthedephosphorylatedserineresidueatposition2808ofRyR2,whichwasconjugatedtokeyholelimpethemocyanin(KLH)bycarbodiimidecrosslinkage |
Isotype | IgG |
Purification | ProteinAaffinitypurified |
Speciesreactivity | TheantibodyrecognisesdephosphorylatedSerine-2808oftheryanodinereceptor.BindingoftheantibodytoitstargetepitopeisblockedinthepresenceofaphosphopeptidecontainingthedephosphoSer2808epitope. |
Testedapplications | WB |
Recommendeddilution | WB1:500 |
Optimization | Optimaldilutionstobedeterminedbyenduser |
Storage | Lyophilisedantibodyisstableat4°Cwhenstoredwithdesiccant.Reconstitutelyophilisedpowderin100µlof18MΩH2O,aliquotandstorefrozenat-80°Cfor1year.Avoidfreeze-thawcycles. |
Storage | Lyophilised:4°C.Reconstituted:-80°C |
Datasheet | A010-35AP |
MSDS | A010-35AP |
Regulatorystatement | Productforresearchuseonly.Notintendedfordiagnosticortherapeuticuse. |