DESCRIPTION:
Afullyvalidated,highlyspecificaffinitypurifiedmousemonoclonalantibodytoSERCAisoforms1and2(anti-SERCA1/2)
SERCA1istheCa2+-pump(109kDa)locatedinthesarcoplasmicreticulum(SR)offast-twitchskeletalmuscleandSERCA2istheCa2+-pumplocatedinthesarcoplasmicreticulum(SR)ofcardiac,smoothandslow-twitchskeletalmuscle.Theenzymetransports2Ca2+fromthecytoplasmintotheSRusingthehydrolysisof1moleculeofATPtopowerthereaction.Thisterminatesmusclecontractionandpromotesmusclerelaxation.MutationsinSERCA1areassociatedwithBrodydisease;mutationsinSERCA2areassociatedwithDarierdiseaseanddownregulationofSERCA2isseeninheartfailure.ThemonoclonalantibodyY/1F4wasproducedbyimmunizingmicewithpurifiedSERCA1:theantibodyinhibitsSERCA1activityuponbinding(Colyeretal.,1989).AntibodyY/1F4recognisesbothSERCA1andSERCA2.
TestedApplications:Westernblot(1:2000dilution),IHCMicroscopy(1:50dilution).Speciesrecognition:Testedonrattissue,butimmunogenicpeptidealignswithMammalian,Avian,AmphibianandFishSERCA
DATASHEET:
Description | AffinitypurifiedmousemonoclonalantibodytoSERCAisoforms1and2 |
Immunogen | Syntheticpeptide(VGNKMFVKGA)correspondingtoaminoacids508-517ofSERCA1,conjugatedtokeyholelimpethemocyanin(KLH)bycarbodiimidecrosslinkage. |
Isotype | IgG |
Purification | ProteinAaffinitypurified |
Speciesreactivity | TestedonRattissue,butimmunogenicpeptidealignswithMammal,Avian,AmphibianandFishSERCA |
Testedapplications | WB |
Recommendeddilution | WB1:2000 |
Optimization | Optimaldilutionstobedeterminedbyenduser |
Storage | Lyophilisedantibodyisstableat4°Cwhenstoredwithdesiccant.Reconstitutelyophilisedpowderin50µlof18MΩH2O,aliquotandstorefrozenat-80°Cfor1year.Avoidfreeze-thawcycles. |
Storage | Lyophilised:4°C.Reconstituted:-80°C |
Datasheet | A010-21AP |
MSDS | A010-21AP |
Regulatorystatement | Productforresearchuseonly.Notintendedfordiagnosticortherapeuticuse. |