DESCRIPTION:
Afullyvalidated,highlyspecificaffinitypurifiedrabbitantibodytothreonine-17phosphorylatedformsofphospholamban
Phospholamban(PLB/PLN)isasmalltransmembraneproteinwhichplaysanimportantroleincontrollingtheactivityofthesarcoplasmicreticulumATPase(SERCA2a)ofcardiacmuscleduringcalciumsequestration.
PhospholambanisphosphorylatedonseparateaminoacidresiduesbycAMP-dependent,andcGMP-dependent(serine-16) andCa2+/CaM-dependent(threonine-17)proteinkinasesinresponsetoβ-adrenergicstimulation.AkthasalsobeenshowntophosphorylateThr-17.Theresultisanincreasedcalciumpumpactivitywhichreducesthetimecourseofthecalciumtransient,increasesthecalciumloadinthesarcoplasmicreticulum,andconsequently,producesalargercalciumtransientatthenextactionpotential.However,alterationinthishomeostaticinteractionhasbeenshowntoresultinheartfailure.
Testedapplications:Westernblot(1:5000dilution).SuppliedaslyophilisedaffinitypurifiedIgG.
DATASHEET:
| Description | AffinitypurifiedRabbitpolyclonalantibody(A010-13AP)tophospholambanphosphoThr-17 |
| Immunogen | Syntheticpeptide(R9SAIRRAST(PO3H2)IEY20)correspondingtoaminoacidssurroundingthephosphorylatedthreonineresidueatposition17ofphospholamban,whichwasconjugatedtokeyholelimpethemocyanin(KLH) |
| Isotype | IgG |
| Purification | ProteinGaffinitypurified.ElutedincitratebufferpH2.8,neutralisedwithTris-base.StABIliseraddedto20%v/v |
| Speciesreactivity | Theantibodyrecognisesmonoandoligomericphospholambanwhenphosphorylatedatthreonine-17.BindingoftheantibodytoitstargetepitopeisblockedinthepresenceofaphosphopeptidecontainingthePLBPhosphoThr-17epitope. |
| Testedapplications | WB |
| Recommendeddilution | WB1:1000 |
| Optimization | Optimaldilutionstobedeterminedbyenduser |
| Storage | Lyophilisedantibodyisstableat4°Cwhenstoredwithdesiccant.Reconstitutelyophilisedpowderin100µlof18MΩH2O,aliquotandstorefrozenat-80°Cfor1year.Avoidfreeze–thawcycles. |
| Storage | Lyophilised:4°CReconstituted:-80°C |
| Datasheet | A010-13AP |
| MSDS | A010-13AP |
| Regulatorystatement | Productforresearchuseonly.Notintendedfordiagnosticortherapeuticuse. |

