DESCRIPTION:
Afullyvalidated,highlyspecificaffinitypurifiedrabbitantibodytothreonine-17phosphorylatedformsofphospholamban
Phospholamban(PLB/PLN)isasmalltransmembraneproteinwhichplaysanimportantroleincontrollingtheactivityofthesarcoplasmicreticulumATPase(SERCA2a)ofcardiacmuscleduringcalciumsequestration.
PhospholambanisphosphorylatedonseparateaminoacidresiduesbycAMP-dependent,andcGMP-dependent(serine-16) andCa2+/CaM-dependent(threonine-17)proteinkinasesinresponsetoβ-adrenergicstimulation.AkthasalsobeenshowntophosphorylateThr-17.Theresultisanincreasedcalciumpumpactivitywhichreducesthetimecourseofthecalciumtransient,increasesthecalciumloadinthesarcoplasmicreticulum,andconsequently,producesalargercalciumtransientatthenextactionpotential.However,alterationinthishomeostaticinteractionhasbeenshowntoresultinheartfailure.
Testedapplications:Westernblot(1:5000dilution).SuppliedaslyophilisedaffinitypurifiedIgG.
DATASHEET:
Description | AffinitypurifiedRabbitpolyclonalantibody(A010-13AP)tophospholambanphosphoThr-17 |
Immunogen | Syntheticpeptide(R9SAIRRAST(PO3H2)IEY20)correspondingtoaminoacidssurroundingthephosphorylatedthreonineresidueatposition17ofphospholamban,whichwasconjugatedtokeyholelimpethemocyanin(KLH) |
Isotype | IgG |
Purification | ProteinGaffinitypurified.ElutedincitratebufferpH2.8,neutralisedwithTris-base.StABIliseraddedto20%v/v |
Speciesreactivity | Theantibodyrecognisesmonoandoligomericphospholambanwhenphosphorylatedatthreonine-17.BindingoftheantibodytoitstargetepitopeisblockedinthepresenceofaphosphopeptidecontainingthePLBPhosphoThr-17epitope. |
Testedapplications | WB |
Recommendeddilution | WB1:1000 |
Optimization | Optimaldilutionstobedeterminedbyenduser |
Storage | Lyophilisedantibodyisstableat4°Cwhenstoredwithdesiccant.Reconstitutelyophilisedpowderin100µlof18MΩH2O,aliquotandstorefrozenat-80°Cfor1year.Avoidfreeze–thawcycles. |
Storage | Lyophilised:4°CReconstituted:-80°C |
Datasheet | A010-13AP |
MSDS | A010-13AP |
Regulatorystatement | Productforresearchuseonly.Notintendedfordiagnosticortherapeuticuse. |